Dr Jakub Sedzinski (Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, University of Copenhagen) will give a talk about Mechanics of basal body patterning during multiciliated cell morphogenesis.
The positioning and dynamics of sub-cellular organelles are crucial for cell and tissue function. A clear example is the patterned arrangement of cilia on the apical surface of multiciliated cells, essential for their synchronized beating. This patterning arises from the complex 2D and 3D dynamics of basal bodies (BBs), membraneless organelles that nucleate and anchor cilia as the apical domain expands. How cells regulate BB dynamics and patterning within the growing apical domain remains unclear. Using quantitative imaging techniques and computational analysis, we show that actin reorganizes into two structures to control BB dynamics. Actin cables transport BBs from the basal to the apical side, while crosslinked actin meshworks distribute and pattern BBs across the apical domain. High-resolution, high-speed imaging reveals that BB movements slow as the actin meshwork becomes more crosslinked. This transition, captured through independent experiments, suggests that actin polymerization and crosslinking distribute and pattern BBs while driving apical domain expansion. These findings highlight how actin reorganization regulates organelle positioning (BBs), influencing tissue-scale functions like fluid flow.
Zoom link here.
Meeting ID: 825 8839 5430
Passcode: TLMJakub20
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